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KMID : 0370220140580030158
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Yakhak Hoeji
2014 Volume.58 No. 3 p.158 ~ p.164
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Quantitative Analysis of the Seventeen Marker Components in Dangguisu-san Using Ultra-performance Liquid Chromatography Coupled to Electrospray Ionization Tandem Mass Spectrometry
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Seo Chang-Seob
Shin Hyeun-Kyoo
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Abstract
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Dangguisu-san is a well-known traditional Korean herbal medicine prescription and has been widely used to treat ecchymosis, blood stagnation, and pain resulting from physical shock in Korea. In this study, an ultra-performance liquid chromatography-electrospray ionization-mass spectrometer method was established for the simultaneous determination of the 17 biomarker components in Dangguisu-san. All analytes were separated on an UPLC BEH C18 (100×2.1 mm, 1.7 μm) column and maintained at 45oC. The mobile phase consisted of two solvent systems, 0.1% (v/v) formic acid in water (A) and acetonitrile (B) by gradient flow. The injection volume was 2.0 μl and the flow rate was 0.3 ml/min with detection at mass spectrometer. Calibration curves of the 17 biomarker components were acquired with r2 values ≥0.9951. The values of limit of detection and quantification of all analytes were 0.02~6.32 ng/ml and 0.05~18.95 ng/ml, respectively. The
amounts of the 17 components in Dangguisu-san sample were 3.17~13,224.50 μg/g.
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KEYWORD
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LC-MS/MS, Dangguisu-san, quantitative analysis
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